Disinfection of drain water

Disinfection of horticulture drain water and adding natural nitrogen with one machine at the same time.

This is what VitalFluid B.V. has been developing during the Clean2fix project. Recirculation of water in greenhouse systems is obligatory to achieve a zero emission of water and achieve the goals of the European water framework directive. One of the challenges is that several pathogens, like viruses, fungi and bacteria can occur in recirculation water and therefore recirculation introduces the risk of spreading pathogens via the irrigation system. Therefore, growers in the horticulture sector often disinfect recirculation water.

Research project
Within this project plasma technology is used to disinfect drain water of a horticulture greenhouse and add natural nitrogen simultaneously. VitalFluid has developed machines that mimic the natural process of lightning. These machines create Plasma Activated Water (PAW) with temporary disinfecting properties and fixated nitrogen.

At Wageningen University & Research different trials have been executed to investigate the possibility of using PAW to disinfect drain water and to add natural nitrogen at the same time. To test the disinfecting capacity, tests were performed on several model plant pathogens (virus ToMV, fungus Fusarium and bacterium Xanthomonas). Concurrently, trials were executed to test what the influence of the process is on the roots and growth of the plants, fruits, and yield.

Disinfection Properties:
– PAW exhibited strong disinfection properties against the fungus Fusarium and bacterium Xanthomonas.
– The log 6 reduction indicates a 99.9999% reduction in the number of viable microorganisms, suggesting that PAW is highly effective in killing these pathogens.

Antiviral Effect:
– The highest dose of PAW resulted in a 7-fold decrease in the number of infectious virus particles, and could be even higher after optimizing the technology.
– This suggests that PAW has antiviral properties, contributing to the reduction of viral contamination.

No Negative Effects on Plants:
– Throughout the trials, there were no negative effects on the growth and conditions of roots, plants, or fruits observed.
– This is a crucial aspect, as it indicates that PAW, at the tested concentrations, is safe for the overall health and development of the fruits.

In summary, the results suggest that PAW is a potent disinfectant against fungi and bacteria, and it also has antiviral properties. Moreover, its application did not adversely affect the growth and health of the plants. These findings may have implications for using PAW as a potential tool for drain water disinfection in agriculture. It’s important to consider further research to validate these results and explore the broader applicability of PAW in different contexts.
D. van der Krieken
Corien Voorburg
Ineke Stijger
S. Breeuwsma
C. Munts
Tommaso Barbagli
Aat van Winkel
Jim van Ruijven MSc
Barbara Eveleens

This project was made possible in part by the European Fund for Regional Development under REACT-EU.

Project: 04062 of Opzuid, Europees programma voor transitie gedreven innovatie